Human Anti-SLC34A2 Recombinant Antibody (TAB-H45)
CAT#: TAB-H45
This product is a human IgG1, κ antibody that can recognize human SLC34A2.
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Anti-Human SLC34A2 Antibody (TAB-H45) in SDS-PAGE
SDS-PAGE analysis of TAB-H45 in non-reduced (lane 1, 2.5ug) and reduced (lane 2, 2.5ug) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Anti-Human SLC34A2 Antibody (TAB-H45) in HPLC
The purity of TAB-H45 was greater than 95% as determined by SEC-HPLC.
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Subcellular Location
Figure 1 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37989/1788_A4_7_cr5971b651a2dbe_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66474/1788_E8_17_cr5971fcb00a73f_selected.jpg
Normal Tissue
Figure 3 High expression in lung
Alveolar cells type Alveolar cells type I I Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37989/141847_A_2_4.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (BEWO, EFO-21, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157765-SLC34A2
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Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG1, κ
- Specificity
- Human SLC34A2
- Species Reactivity
- Human
- Applications
- ELISA, FC, IP, FuncS, IF, Neut, ICC
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunoprecipitation, Functional Assay, Immunofluorescence, Neutralization, Immunocytochemistry.
Target
- Alternative Names
- PULAM; NPTIIb; NAPI-3B; NAPI-IIb; sodium-dependent phosphate transport protein 2B; sodium/phosphate cotransporter 2B; solute carrier family 34 (sodium phosphate), member 2; solute carrier family 34 (type II sodium/phosphate cotransporter), member 2; type II sodium-dependent phosphate transporter 3b
- Gene ID
- 10568
- UniProt ID
- O95436
REVIEWS AND Q&AS
CITATIONS
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Aids in Metastasis Research
The antibody is invaluable in metastasis research, as it helps analyze how changes in SLC34A2 expression impact cancer dissemination and aggressiveness, informing potential interventions.
Excellent for Functional Assays
In functional assays, this antibody performs exceptionally well. It provides clear insights into SLC34A2's impact on cellular processes, critical for understanding its role in cancer cell survival and proliferation.
High Sensitivity in Detection
We've observed excellent sensitivity when using this antibody, enabling the detection of SLC34A2 even in low-grade tumors. This sensitivity supports our experimental explorations in early-stage cancer detection and treatment research.
Q&As
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What is the main application of the Anti-SLC34A2 Recombinant Antibody (TAB-H45)?
A: This antibody is primarily used in cancer research, specifically in studying the expression and function of SLC34A2, a sodium-dependent phosphate transport protein. It is utilized in applications such as immunofluorescence (IF), immunoprecipitation (IP), ELISA, and functional assays to analyze its role in cellular metabolism and its potential as a cancer biomarker.
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How does SLC34A2 contribute to oncological research?
A: SLC34A2 is often overexpressed in certain cancers, making it a valuable target for understanding tumor biology and developing targeted cancer therapies. By investigating its expression and role, researchers can gain insights into tumor growth, metastasis, and potential therapeutic interventions that target this transporter.
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What challenges does this antibody address in experimental setups?
A: It mitigates issues related to nonspecific binding and lack of sensitivity, providing precise and clear data in complex samples, which is vital in cancer research where detecting subtle changes in protein expression is crucial.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-H45, RRID: AB_3112032)
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Biosimilar Overview
Please refer to Lifastuzumab Vedotin Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Lifastuzumab Vedotin.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| HPAB-M0659-YC-F(E) | Human Anti-SLC34A2 Recombinant Antibody; Fab Fragment (HPAB-M0659-YC-F(E)) | FC, IHC, FuncS | Humanized Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1661z-S(P) | Recombinant Anti-Human SLC34A2 Antibody scFv Fragment | FC, IP, FuncS | scFv |
| HPAB-M0659-YC-S(P) | Human Anti-SLC34A2 Recombinant Antibody; scFv Fragment (HPAB-M0659-YC-S(P)) | FC, IHC, FuncS | Humanized scFv |
| HPAB-M0281-YC-S(P) | Human Anti-SLC34A2 Recombinant Antibody; scFv Fragment (HPAB-M0281-YC-S(P)) | FuncS | Human scFv |
| HPAB-M0282-YC-S(P) | Human Anti-SLC34A2 Recombinant Antibody; scFv Fragment (HPAB-M0282-YC-S(P)) | FC, FuncS | Human scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-467MZ | Mouse Anti-SLC34A2 Recombinant Antibody (TAB-467MZ) | FC, Inhib | Mouse IgG1 |
| TAB-467MZ-S(P) | Mouse Anti-SLC34A2 Recombinant Antibody; scFv Fragment (TAB-467MZ-S(P)) | SDS-PAGE, IHC, WB | Mouse scFv |
| TAB-467MZ-F(E) | Mouse Anti-SLC34A2 Recombinant Antibody; Fab Fragment (TAB-467MZ-F(E)) | SDS-PAGE, IHC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-468MZ | Human Anti-SLC34A2 Recombinant Antibody (TAB-468MZ) | FC, Inhib | Humanized antibody |
| TAB-468MZ-S(P) | Human Anti-SLC34A2 Recombinant Antibody; scFv Fragment (TAB-468MZ-S(P)) | SDS-PAGE, IHC, WB | Humanized scFv |
| TAB-468MZ-F(E) | Human Anti-SLC34A2 Recombinant Antibody; Fab Fragment (TAB-468MZ-F(E)) | SDS-PAGE, IHC, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0341LY | Human Anti-SLC34A2 Recombinant Antibody (HPAB-0341LY) | ELISA, Block | Humanized IgG1 |
| HPAB-M0659-YC | Human Anti-SLC34A2 Recombinant Antibody (HPAB-M0659-YC) | FC, IHC, FuncS | Humanized IgG |
| HPAB-M0281-YC | Human Anti-SLC34A2 Recombinant Antibody (HPAB-M0281-YC) | FuncS | Human IgG1 |
| HPAB-M0282-YC | Human Anti-SLC34A2 Recombinant Antibody (HPAB-M0282-YC) | FC, FuncS | Human IgG1 |
| HPAB-N0246-YC | Human Anti-SLC34A2 Recombinant Antibody (HPAB-N0246-YC) | FuncS | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H45 | Afuco™ Anti-SLC34A2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H45) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY167 | Human Anti-SLC34A2 scFv-Fc Chimera (VS-0425-FY167) | IHC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC70 | Recombinant Anti-SLC34A2 Vesicular Antibody, EV Displayed (VS-0425-YC70) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
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| VS-0525-XY6677 | Anti-SLC34A2 Immunohistochemistry Kit | IHC |
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